Eikon's technology platform combines live-cell
super-resolution microscopy and cutting-edge engineering

Cellular regulatory circuits typically exploit rapid, dynamic interactions among protein molecules. Yet much of the scientific community’s understanding of biology is based on visualization tools that provide static snapshots of a moment in time.


Eikon has developed proprietary, purpose-built platforms that permit precise characterization of protein interactions in living cells with exceptional spatial and temporal resolution. These tools provide a means of examining fleeting but essential interactions among individual protein molecules in living cells. We believe this knowledge will enable the discovery of innovative treatments that will improve and extend life.

Super-Resolution
Fluorescence Microscopy

The invention of fluorescence microscopy made it possible for scientists to visualize cell biology, but until recently, discoveries were limited by the wavelength of light. Nobel prize-winning super-resolution fluorescence microscopy — the foundation of Eikon’s platform — is a disruptive advancement that enables scientists to visualize individual proteins.

Single Particle Imaging

Eikon uses proprietary, purpose-built microscopes coupled with robotic cell manipulation systems and high-performance computing to visualize the movement of selected proteins in living cells.(ref) We fluorescently label an endogenous protein target and image individual proteins with 10 nm spatial resolution and 10 millisecond time resolution.

Movement of a Protein in the Cell Membrane

Single Particle Tracking (SPT)

Each time series reveals quantifiable, multi-dimensional characterization of protein dynamics, including the trajectory of each protein and its characteristic jump length, angular movements, on-rates/off-rates as well as residence times for binding events. We extract these data using proprietary software packages, purpose-built for scale and utilization in drug discovery.

Industrialization

The power of Eikon's platform is its scale. We have engineered a fully automated system that can run thousands of SPT assays and process the resulting data each day, enabling high throughput screening of SPT biology. Our automated systems reach beyond SPT and are applied broadly across the drug discovery process.

Learn how Eikon uses SPT in drug discovery

References on the development of SPT technology

Robust model-based analysis of single-particle tracking experiments with Spot-On. Hansen AS, Woringer M, Grimm JB, Lavis LD, Tjian R, Darzacq X (2018) Elife, 7:e33125.

Bright photoactivatable fluorophores for single-molecule imaging. Grimm, J. B., English, B. P., Choi, H., Muthusamy, A. K., Mehl, B. P., Dong, P., Brown, T. A., Lippincott-Schwartz, J., Liu, Z., Lionnet, T., & Lavis, L. D. (2016). Nature methods, 13(12), 985–988.

A general method to improve fluorophores for live-cell and single-molecule microscopy. Grimm, J. B., English, B. P., Chen, J., Slaughter, J. P., Zhang, Z., Revyakin, A., Patel, R., Macklin, J. J., Normanno, D., Singer, R. H., Lionnet, T., & Lavis, L. D. (2015). Nature methods, 12(3), 244–250.

Imaging intracellular fluorescent proteins at nanometer resolution. Eric Betzig, George H Patterson, Rachid Sougrat, O Wolf Lindwasser, Scott Olenych, Juan S Bonifacino, Michael W Davidson, Jennifer Lippincott-Schwartz, Harald F Hess. (2006) Science 313, 1642-5. 10.1126/science.1127344.